A highly efficient and sustainable photoabsorber in solar-driven seawater desalination and wastewater purification.

Producing freshwater from seawater and wastewater is of great importance through interfacial solar steam generation (ISSG). Herein, the three-dimensional (3D) carbonized pine cone, CPC1, was fabricated via a one-step carbonization process as a low-cost, robust, efficient, and scalable photoabsorber for the ISSG of seawater as well as a sorbent/photocatalyst for use in wastewater purification. Taking advantage of the large solar-light-harvesting ability of CPC1 due to the presence of carbon black layers on the 3D structure, its inherent porosity, rapid water transportation, large water/air interface, and low thermal conductivity, a conversion efficiency of 99.8% and evaporation flux of 1.65 kg m-2 h-1 under 1 sun (kW m-2) illumination were achieved. After carbonization of the pine cone, its surface becomes black and rough, which leads to an increase in its light absorption in the UV-Vis-NIR region. The photothermal conversion efficiency and evaporation flux of CPC1 did not change significantly during 10 evaporation-condensation cycles. CPC1 exhibited good stability under corrosive conditions without significant change in its evaporation flux. More importantly, CPC1 can be used to purify seawater or wastewater by the removal of organic dyes as well as by the reduction of polluting ions, like nitrate ions in sewage.

The Prevalence of Andropause and Its Relationship With Sexual Quality of Life Among Older Iranian Men.

Available evidence indicates insufficient knowledge about the status of andropause and sexual quality of life among Iranian older men. The study aimed to investigate the prevalence of andropause and its relationship with sexual quality among older adults. This descriptive-analytical study was conducted among 576 older people referred to urban health centers in Mashhad, Iran. The eligible samples were selected through the cluster sampling method. To collect data, the male andropause symptoms' self-assessment questionnaire and the sexual quality of life-male were used. Forty-seven of the respondents (n = 271) were diagnosed with a "moderate" level of andropause. A strong negative correlation was identified between the sexual quality of life and the severity of andropause (r = -.366, p < .001). Sexual quality of life and andropause was also affected by age, marital status, health status, and exercise. Thirty-six percent of the changes in the quality of sexual life of older men were influenced by the independent variables (adjusted R2 = .36; R2 = .40; R = .63). The findings indicated that andropause has highly prevailed among the participants. There was a meaningful association between andropause and sexual quality of life among older men. Further studies are recommended to investigate sexual orientation qualitatively and to discover other factors influencing andropause among adult men.

Experimental and modeling studies of the effects of nanoclay on the oil behaviors in a water-sand system.

When oil is released into the oceans, spilled oil may get to the shoreline driven by wind and wave. This study comprehensively explored the effects of bentonite nanoclay on the oil behaviors in a water-sand system from both experimental and modeling perspectives. Four factors including nanoclay concentration, temperature, salinity, and pH have been studied. The increasing nanoclay concentration resulted in the decrease in remaining oil on sand. Higher temperature and salinity were associated with less residual oil on sand in the presence of nanoclay. The lower residual oil on sand with coexisting nanoclay was found to be at pH 7. The factorial analysis results indicated that the nanoclay concentration showed the most significant impact among these factors. Miscibility modeling results showed an increasing temperature was favorable to the nanoclay miscibility. Moreover, the effect of nanoclay on oil behavior was further revealed through the dynamic simulation, in which it can be seen the nanoclay could penetrate into oil droplets and promote the oil detachment from solid substrate. The results of this study can help understand the role of fine particles in the fate and transport of oil on shoreline and support the risk assessment and response planning after oil spill.

Evaluation of a Persian version of the Adelaide driving self-efficacy scale among Iranian older adults.

OBJECTIVE: Our aim in the present study was to estimate the psychometric properties of the full-length Adelaide driving self-efficacy scale (ADSES) for use among community-based resident older adults in Tehran, Iran. METHODS: We recruited older adults (60+ years) from various sampling units nested in the Tehran district's general urban population (20 subjects/questionnaire-item). The questionnaire was translated and back-translated by using recommended pathways. Multiple forms of validity and reliability, including Cronbach alpha, were estimated. Also, we measured intra-class correlation coefficient, and did confirmatory factor analysis (CFA). RESULTS: A total of 243 participants (mean age: 65.8, 95%CI 65.4-66.3) met our inclusion criteria. For ADSES, the alpha coefficient was 0.77, the intraclass correlation coefficient was 0.97 (95% CI: 0.95-0.98), and the average item-test correlation was 0.67. Upon CFA, we found a 0.95 comparative fit index, a coefficient of determination = 92.6%, and standardized size of the residual = 0.04. CONCLUSION: Our Persian language ADSES was found to have adequate validity and factor structure parameters for evaluating driving self-efficacy among community-based older adults in a non-western context. Our questionnaire is an essential first step toward evaluating driving self-efficacy among older adults, especially where no such tool is available, to help develop driving self-efficacy as a healthy aging measure.

Insights into the Host Specificity of a New Oomycete Root Pathogen, Pythium brassicum P1: Whole Genome Sequencing and Comparative Analysis Reveals Contracted Regulation of Metabolism, Protein Families, and Distinct Pathogenicity Repertoire.

Pythium brassicum P1 Stanghellini, Mohammadi, Förster, and Adaskaveg is an oomycete root pathogen that has recently been characterized. It only attacks plant species belonging to Brassicaceae family, causing root necrosis, stunting, and yield loss. Since P. brassicum P1 is limited in its host range, this prompted us to sequence its whole genome and compare it to those of broad host range Pythium spp. such as P. aphanidermatum and P. ultimum var. ultimum. A genomic DNA library was constructed with a total of 374 million reads. The sequencing data were assembled using SOAPdenovo2, yielding a total genome size of 50.3 Mb contained in 5434 scaffolds, N50 of 30.2 Kb, 61.2% G+C content, and 13,232 putative protein-coding genes. Pythium brassicum P1 had 175 species-specific gene families, which is slightly below the normal average. Like P. ultimum, P. brassicum P1 genome did not encode any classical RxLR effectors or cutinases, suggesting a significant difference in virulence mechanisms compared to other oomycetes. Pythium brassicum P1 had a much smaller proportions of the YxSL sequence motif in both secreted and non-secreted proteins, relative to other Pythium species. Similarly, P. brassicum P1 had the fewest Crinkler (CRN) effectors of all the Pythium species. There were 633 proteins predicted to be secreted in the P. brassicum P1 genome, which is, again, slightly below average among Pythium genomes. Pythium brassicum P1 had only one cadherin gene with calcium ion-binding LDRE and DxND motifs, compared to Pythium ultimum having four copies. Pythium brassicum P1 had a reduced number of proteins falling under carbohydrate binding module and hydrolytic enzymes. Pythium brassicum P1 had a reduced complement of cellulase and pectinase genes in contrast to P. ultimum and was deficient in xylan degrading enzymes. The contraction in ABC transporter families in P. brassicum P1 is suggested to be the result of a lack of diversity in nutrient uptake and therefore host range.

Morphological and Rheological Properties of PLA, PBAT, and PLA/PBAT Blend Nanocomposites Containing CNCs.

Morphological and rheological properties of poly(lactic acid), PLA (semicrystalline and amorphous), and poly(butylene adipate-co-terephthalate), PBAT, and their blends (75 wt%/25 wt%; PLA/PBAT) were investigated in the presence of cellulose nanocrystals (CNCs) prepared from solution casting followed by melt mixing. For the solution casting step, the CNCs were either incorporated into the matrix, the dispersed phase, or both. The dispersion and distribution of the CNCs in the neat polymers and localization in their blends were analyzed via scanning electron microscopy (SEM) and atomic force microscopy (AFM). The highly dispersed CNCs in the solution cast nanocomposites were agglomerated after melt mixing. In the blends with 1 wt% CNCs, the nanoparticles were mostly localized on the surface of the PBAT droplets irrespective of their initial localization. The rheological behavior of the single polymer matrix nanocomposites and their blends was determined in dynamic and transient shear flow in the molten state. Upon melt mixing the complex viscosity and storage modulus of the solution cast nanocomposites decreased markedly due to re-agglomeration of the CNCs. Under shearing at 0.1 s-1, a significant droplet coalescence was observed in the neat blends, but was prevented by the presence of the CNCs at the interface in the blend nanocomposites.

Self-Healing Potential and Phase Evolution Characterization of Ternary Cement Blends.

The autogenous self-healing of cementitious material micro-cracks might lead to the service-life extension of structures. However, most of its aspects are still unknown. This paper investigates the self-healing capacity of ternary cement blends including metakaolin (MK), ground granulated blast-furnace slag (BFS), limestone (LS), and siliceous filler (F). Morphology and healing precipitation patterns were studied through the optical microscopy of artificial micro-cracks, global healing product mass monitoring, and XRD and TGA used to identify and quantify mineral formation. The self-healing potential index is introduced based on the mass measurements. It was found that the formulation containing 10% MK presented the highest healing potential at an early age (<28 days), while the formulations containing 20% BFS with 10% LS/F showed a higher healing potential at an older age (cracked after 28 days of curing). Calcite, C-S-H, and portlandite were found to be the main healing products alongside specific formulation-dependent compounds, and it was observed that the calcite's relative quantity generally increased with time. Finally, the evolution of the self-healing product phases was accurately monitored through XRD and TGA measurements.

Tick Ectoparasites of Animals in Borderline of Iran-Iraq and Their Role on Disease Transmission.

BACKGROUND: Since ticks are potent vectors of various diseases, identification of these species are clinically important to protect the public health and control veterinary problems in the communities. We aimed to figure out the frequency of ticks on cows, goats, sheep, lambs, turtles and also obscure hosts in Kurdistan Province, bordered with Iraq June 2012 to May 2013. METHODS: The hosts were selected randomly and examined individually for tick infestation. In case of infestation, ticks were collected using forceps and then preserved in 70% ethyl alcohol. All collected specimens were preserved in tubes and relative information was recorded and then identified based on morphological characteristics. RESULTS: Totally, 1209 ticks were collected. The prevalence of ticks on cows, sheep, goats, lambs, turtles, poultry and obscure hosts was 11.33%, 55.41%, 6.53%, 5.95%, 0.9%, 8.02% and 11.82% respectively. The mean number of ticks on each animal was 1.6. Number of 5 genera, including Rhipicephalus, Argas, Ornithodoros, Hyalomma and Haemaphysalis and 9 species; including R. sanguineus (60.05%), R. bursa (0.08), Hy. anatolicum (12.33), Hy. asiaticum (1.49), Hy. aegyptium (0.91), Hy. marginatum (0.08), Haemaphysalis parva (4.22), Hyalomma sp. (0.99), Ornithodoros lahorensis (11.83), and Argas persicus (8.02) were identified. CONCLUSION: The most abundant species in this study area was Rh. sanguineus (60.05%). Due to high prevalence of tick specimens and a variety of collected species from sheep (55.41%), the vaccination of sheep and control of tick vectors are recommended.

Siderophore-mediated iron acquisition influences motility and is required for full virulence of the xylem-dwelling bacterial phytopathogen Pantoea stewartii subsp. stewartii.

Iron is a key micronutrient for microbial growth but is often present in low concentrations or in biologically unavailable forms. Many microorganisms overcome this challenge by producing siderophores, which are ferric-iron chelating compounds that enable the solubilization and acquisition of iron in a bioactive form. Pantoea stewartii subsp. stewartii, the causal agent of Stewart's wilt of sweet corn, produces a siderophore under iron-limiting conditions. The proteins involved in the biosynthesis and export of this siderophore are encoded by the iucABCD-iutA operon, which is homologous to the aerobactin biosynthetic gene cluster found in a number of enteric pathogens. Mutations in iucA and iutA resulted in a decrease in surface-based motility that P. stewartii utilizes during the early stages of biofilm formation, indicating that active iron acquisition impacts surface motility for P. stewartii. Furthermore, bacterial movement in planta is also dependent on a functional siderophore biosynthesis and uptake pathway. Most notably, siderophore-mediated iron acquisition is required for full virulence in the sweet corn host, indicating that active iron acquisition is essential for pathogenic fitness for this important xylem-dwelling bacterial pathogen.

Pythium brassicum sp. nov.: A Novel Plant Family-Specific Root Pathogen.

Roots of stunted broccoli plants (Brassica oleracea) from the Palo Verde Valley, CA, were observed at various stages of decay. A species of Pythium with large spiny oogonia was microscopically observed and consistently isolated from decayed roots. Isolates produced spherical, intercalary sporangia (average 34.5 µm in diameter) and aplerotic oospores (average 37.3 µm in diameter) in oogonia (average 47.4 µm in diameter) bearing numerous conical spines (average 8.5 µm in height and 5.5 µm basal width) with blunt apices. A representative broccoli isolate (P1) had a 99% internal transcribed spacer (ITS) sequence similarity with Pythium jasmonium (nom. inval., GenBank AF216654.1), a species which has not been formally described. Three other accessions in GenBank also carry the specific epithet of P. jasmonium and were originally isolated from diseased plants in the family Brassicaceae. In our study, these isolates were pathogenic on broccoli and morphologically similar to P1. P1 was pathogenic to 10 cultivated and 12 wild plants in the family Brassicaceae but not to 18 species of cultivated plants belonging to nine other plant families. Mycelial growth of our isolates occurred between <10 and 35°C, with an optimum of 25°C (maximum growth rate 25 mm/day). Our broccoli isolates are related to other species in subclade B of clade J. Members of subclade B include P. mastophorum, P. uncinulatum, P. buismaniae, P. polymastum, and P. megalacanthum. However, the broccoli isolates, in addition to all those in GenBank that carry the specific epithet of P. jasmonium, possess unique ITS, ß-tubulin, and cox1 sequences that are sufficiently different from other species in subclade B to justify status as a new species. We propose that isolates previously designated as P. jasmonium (nom. inval.) be renamed as P. brassicum sp. nov. based on their apparent plant family-specific pathogenicity.

Biological role of pigment production for the bacterial phytopathogen Pantoea stewartii subsp. stewartii.

Pantoea stewartii subsp. stewartii, the causal agent of Stewart's wilt of sweet corn, produces a yellow carotenoid pigment. A nonpigmented mutant was selected from a bank of mutants generated by random transposon mutagenesis. The transposon insertion site was mapped to the crtB gene, encoding a putative phytoene synthase, an enzyme involved in the early steps of carotenoid biosynthesis. We demonstrate here that the carotenoid pigment imparts protection against UV radiation and also contributes to the complete antioxidant pathway of P. stewartii. Moreover, production of this pigment is regulated by the EsaI/EsaR quorum-sensing system and significantly contributes to the virulence of the pathogen in planta.

Pantoea stewartii subsp. stewartii produces an endoglucanase that is required for full virulence in sweet corn.

Pantoea stewartii subsp. stewartii, a xylem-dwelling bacterium, is the causal agent of Stewart's wilt and blight of sweet corn. The goal of this study was to characterize the only gene in the P. stewartii subsp. stewartii genome predicted to encode an endoglucanase (EGase); this gene was designated engY. Culture supernatants from P. stewartii subsp. stewartii and Escherichia coli expressing recombinant EngY protein possessed both EGase and xylanase activities. Deletion of engY abolished EGase and xylanase activity, demonstrating that EngY appears to be the major EGase or xylanase produced by P. stewartii subsp. stewartii. Most importantly, our results show that EngY contributes to movement in the xylem and disease severity during the wilting phase of Stewart's wilt but is not required for water-soaked lesion formation.

Hospital waste management status in Iran: a case study in the teaching hospitals of Iran University of Medical Sciences.

Hospital waste materials pose a wide variety of health and safety hazards for patients and healthcare workers. Many of hospitals in Iran have neither a satisfactory waste disposal system nor a waste management and disposal policy. The main objective of this research was to investigate the solid waste management in the eight teaching hospitals of Iran University of Medical Sciences. In this cross-sectional study, the main stages of hospital waste management including generation, separation, collection, storage, and disposal of waste materials were assessed in these hospitals, located in Tehran city. The measurement was conducted through a questionnaire and direct observation by researchers. The data obtained was converted to a quantitative measure to evaluate the different management components. The results showed that the waste generation rate was 2.5 to 3.01 kg bed(-1) day(-1), which included 85 to 90% of domestic waste and 10 to 15% of infectious waste. The lack of separation between hazardous and non-hazardous waste, an absence of the necessary rules and regulations applying to the collection of waste from hospital wards and on-site transport to a temporary storage location, a lack of proper waste treatment, and disposal of hospital waste along with municipal garbage, were the main findings. In order to improve the existing conditions, some extensive research to assess the present situation in the hospitals of Iran, the compilation of rules and establishment of standards and effective training for the personnel are actions that are recommended.

Autoinducer-2 of the fire blight pathogen Erwinia amylovora and other plant-associated bacteria.

Autoinducers are important for cellular communication of bacteria. The luxS gene has a central role in the synthesis of autoinducer-2 (AI-2). The gene was identified in a shotgun library of Erwinia amylovora and primers designed for PCR amplification from bacterial DNA. Supernatants of several Erwinia amylovora strains were assayed for AI-2 activity with a Vibrio harveyi mutant and were positive. Many other plant-associated bacteria also showed AI-2 activity such as Erwinia pyrifoliae and Erwinia tasmaniensis. The luxS genes of several bacteria were cloned, sequenced, and complemented Escherichia coli strain DH5alpha and a Salmonella typhimurium mutant, both defective in luxS, for synthesis of AI-2. Assays to detect AI-2 activity in culture supernatants of several Pseudomonas syringae pathovars failed, which may indicate the absence of AI-2 or synthesis of another type. Several reporter strains did not detect synthesis of an acyl homoserine lactone (AHL, AI-1) by Erwinia amylovora, but confirmed AHL-synthesis for Erwinia carotovora ssp. atroseptica and Pantoea stewartii.

The Bt gene cry2Aa2 driven by a tissue specific ST-LS1 promoter from potato effectively controls Heliothis virescens.

Expression of the Cry2Aa2 protein was targeted specifically to the green tissues of transgenic tobacco Nicotiana tabacum cv. Xanthi plants. This deployment was achieved by using the promoter region of the gene encoding the Solanum tuberosum leaf and stem specific (ST-LS1) protein. The accumulated levels of toxin in the leaves were found to be effective in achieving 100% mortality of Heliothis virescens larvae. The levels of Cry2Aa2 expression in the leaves of these transgenic plants were up to 0.21% of the total soluble proteins. Bioassays with R1 transgenic plants indicated the inheritance of cry2Aa2 in the progeny plants. Tissue-specific expression of the Bt toxin in transgenic plants may help in controlling the potential occurrence of insect resistance by limiting the amount of toxin to only predated tissues. The results reported here validate the use of the ST-LS1 gene promoter for a targeted expression of Bt toxins in green tissues of plants.

Induced chitinase activity in resistant wheat leaves inoculated with an incompatible race of Puccinia striiformis f. sp. tritici, the causal agent of yellow rust disease.

Chitinase specific activity was measured spectrophotometrically in wheat leaf tissues during the compatible and incompatible interactions with Puccinia striiformis f. sp. tritici, the causal agent of yellow rust disease. The wheat cultivar, Federation* 4/Kavkaz, was inoculated with virulent (134E134A+) or avirulent (4EOA+) races of P. striiformis f. sp. tritici in the first leaf stage. The results showed that chitinase activity pattern was similar in both compatible and incompatible interactions up to 72 hrs after inoculation. However, the specific activity increased rapidly in the incompatible reaction thereafter. In susceptible reaction, chitinase activity gradually declined after 72 hrs post-inoculation reaching a level similar to that in the control plants two weeks after inoculation. Chitinase specific activity in resistance response was at least three times greater than that in the susceptible reaction two weeks following the inoculation. Electrophoresis of native polyacrylamide gel impregnated with 0.1% (w/v) glycol chitin as the substrate revealed the presence of eight chitinase isoforms with relative electrophoretic mobility (Rm) values ranging from 0.11 to 0.64 in the resolving gel.